NAD+
Purchase NAD⁺ Research Compound
Nicotinamide Adenine Dinucleotide (NAD⁺) is a naturally occurring coenzyme found in all living cells and extensively studied in metabolic biology, aging research, and cellular energetics for its fundamental role as an electron carrier and signaling molecule. This essential biomolecule serves as a critical research tool for investigating mitochondrial function, DNA repair mechanisms, and sirtuin-mediated longevity pathways in preclinical models.
NAD⁺ Identity & Structural Overview
NAD⁺ is a dinucleotide composed of adenosine monophosphate (AMP) and nicotinamide mononucleotide (NMN) linked by a phosphoanhydride bond. Its oxidized form (NAD⁺) and reduced form (NADH) constitute a redox pair central to cellular metabolism. The nicotinamide ring serves as the active site for hydride transfer in enzymatic oxidation-reduction reactions across glycolysis, the citric acid cycle, and oxidative phosphorylation pathways.
Mechanism of Action
Preclinical research demonstrates that NAD⁺ functions as an essential cofactor for over 500 enzymatic reactions, including those catalyzed by sirtuins (SIRT1–SIRT7), poly(ADP-ribose) polymerases (PARPs), and cyclic ADP-ribose synthases (CD38/CD157). Studies have documented its role in regulating mitochondrial biogenesis, modulating inflammatory signaling, supporting genomic stability through DNA strand break repair, and influencing circadian rhythm gene expression in cellular and animal model systems.
Key Research Findings
Foundational work by Guarente et al. established the link between NAD⁺-dependent sirtuin activation and lifespan extension in model organisms, forming the basis for subsequent aging biology research. Studies by Verdin et al. further documented declining intracellular NAD⁺ concentrations as a hallmark of cellular aging, with preclinical research demonstrating that restoration of NAD⁺ availability supports mitochondrial function and metabolic homeostasis in aged tissue preparations and animal models.
Product Specifications
Form: Lyophilized powder (synthetic, high-purity)
Chemical Name: Nicotinamide Adenine Dinucleotide (oxidized form)
Molecular Weight: 663.4 Da
Storage: −20°C; protect from light; unopened shelf life of 24 months
Reconstitution: Sterile water or phosphate-buffered saline
Purity: >95% (HPLC verified); endotoxin <1 EU/μg
Research Use Only
For preclinical research purposes only. Not intended for human or veterinary use. All handling must adhere to institutional biosafety committee guidelines.
